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Vascular storage parenchyma cells of carrot roots were treated with methanol solutions of radicinin and epiradicinol produced by Alternaria radicina and with alternariol and alternariol methyl ether produced by A. alternata at concentrations of 25 µg/ml and 250 µg/ml, as well as culture filtrates of both fungi. Cell ultrastructure was observed by TEM. No visible changes were noted after treatment...
The effects of the exogenous growth regulators abscisic and gibberellic acids (ABA and GA3) on the activity of arginine (ADC) and ornithine decarboxylases (ODC) during dormancy breaking were studied in beech seed. During cold-stratification at 3°C, ADC and ODC activity increased rapidly starting from week 7 in embryo axes and week 8 in cotyledons. At 15°C, ADC activity was higher than ODC activity...
A simplified cDNA cloning protocol was elaborated, easily scalable to very different sizes of plant tissue. The mRNA fraction was extracted from a single wheat kernel with oligo dT magnetic beads. The solid-phase mRNA was transcribed into single-strand cDNA by reverse transcription. Then DNA tags were incorporated into the ds cDNA fragments prior to PCR amplification. The amplified DNA was ligated...
Polyphenol oxidases (PPOs) reveal a range of forms and occur in all plants and crops. PPOs are comprised of three enzymes (catecholase, laccase, cresolase) with very different activities and specificities. Cresolase has a dualistic form (cresolase is only in plants and tyrosinase is only in animals and microorganisms). Very often in the literature the generic word "PPO" is used inappropriately...
The development and (ultra)structure of vascular tissue was studied in Lupinus luteus L. mature root nodules 25 and 60 days after inoculation. In the proximal part of the nodule vascular trace, extensive growth took place to match the secondary growth of the root. Development of bacteroid and vascular tissues was correlated. The non-anastomosing nodule vascular bundles elongated and branched due to...
In this study, somatic chromosome counts were determined in 302 individuals from 43 Allium przewalskianum populations; 90 were diploids (2n = 16, 16 + 1B, 16 + 2B) and 212 were tetraploids (2n = 32). Of the 43 populations, five were selected for karyotype analysis. Among them, the diploid plants have two karyotypes: 2n = 2x = 14m + 2st (2SAT) and 2n =2x = 8m + 6sm + 2st (2SAT). The tetraploid has...
Reaumuria soongorica (Pall.) Maxim., a perennial semishrub, is able to survive almost complete tissue dehydration when water is withheld from it, and then the stem can rehydrate on rewatering. In this work, a series of experiments were conducted to characterize the drought-induced changes in two-year-old Reaumuria soongorica. The plants were subjected to dehydration by withholding water for 15 days...
In a pot culture experiment, the effect of calcium chloride (CaCl2) as an ameliorating agent on sodium chloride (NaCl) stress was studied in Dioscorea rotundata plants. Plants were raised in pots and exposed to salinity stress (80 mM NaCl) with or without 5 mM CaCl2. NaCl-stressed plants showed decreased protein and total sugars, and increased free amino acid and proline content. When NaCl treatment...
Anatomical and palynological features of E. algidum Bieb., E. palustre L., E. ponticum Hausskn., E. confusum Hausskn., E. hirsutum L. and E. montanum L. collected from NE Anatolia were examined and evaluated by numerical analysis in order to determine the taxonomic value of the observed internal peculiarities. Features related to pollen shape and ornamentation, idioblast distribution, number of palisade...
This is the first report of somatic embryogenesis from leaves of two strawberry cultivars (Selva and Comarosa) cultured on MS medium containing picloram. Maximum embryogenesis was induced using 2 mg/l picloram. Globular stage embryos developed into cotyledonary ones after transfer to hormone-free media containing 2%, 4%, 6%, 8% and 10% (w/v) sucrose. Increasing sucrose concentrations in culture media...
Phylogenetic relationships among wild Triticum and Aegilops species, bread wheat (Triticum aestivum) cultivars, and durum wheat (T. turgidum) cultivars were investigated using random amplified polymorphic DNA (RAPD) technique. Fourteen RAPD primers generated 328 polymorphic bands in 22 wheat species/cultivars which have the same or different genomes. DNA fragment size ranged from 290 bp to 2570 bp...
The ontogeny and (ultra)structure of vascular tissue in Lupinus luteus L. root nodules were studied by light and transmission electron microscopy in juvenile nodule primordia up to the 11th day after inoculation. Vascular meristem originated from centripetally dedifferentiated root cortical parenchyma, endodermis and pericycle. The vascular trace was formed between bacteroid tissue initials and the...
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